anti phospho histone h2ax Search Results


human phospho-histone h2ax (s139) antibody  (Bio-Techne corporation)
99
Bio-Techne corporation human phospho-histone h2ax (s139) antibody
Human Phospho Histone H2ax (S139) Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human phospho-histone h2ax (s139) antibody/product/Bio-Techne corporation
Average 99 stars, based on 1 article reviews
human phospho-histone h2ax (s139) antibody - by Bioz Stars, 2026-02
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phospho-histone h2ax (ser139) (γh2ax  (Merck & Co)
90
Merck & Co phospho-histone h2ax (ser139) (γh2ax
MGMT fusions protect from TMZ induced damage. a Clonogenic survival assay of U251 clones expressing MGMT fusions exposed to O 6 -BG (100μM) or/and TMZ (100μM) for 12 days. U251sgMSH6 cells are shown as control for TMZ resistance independently from MGMT. b Cell cycle distribution of U251 MGMT fusion expressing cells in presence of O 6 -BG (100μM) or/and TMZ (100μM) for 72h, measured by propidium iodide (PI) staining and FACS. U251 sgCtrl and sgMSH6 are shown as control. c High-throughput microscopy mediated quantification of cell cycle distribution at 48h after treatment. See methods for details. d Quantification of the percentage of cells in (c). Data are from a representative experiment repeated in triplicate and presented as mean (technical replicate n=3) and standard deviation. e - f High-throughput microscopy mediated quantification of <t>γH2AX</t> intensity levels and 53BP1 foci in U251 cells expressing the MGMT fusions after 48h of treatment with 100μM of the indicated drugs. U251sgCtrl and sgMSH6 were included as controls. Data are representative of three independent experiments. Student’s t test: *** P < 0.001, ** P < 0.01, * P < 0.05, ns = not significant; A.U., arbitrary unit.
Phospho Histone H2ax (Ser139) (γh2ax, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho-histone h2ax (ser139) (γh2ax/product/Merck & Co
Average 90 stars, based on 1 article reviews
phospho-histone h2ax (ser139) (γh2ax - by Bioz Stars, 2026-02
90/100 stars
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phosphorylated γh2ax  (Enzo Biochem)
90
Enzo Biochem phosphorylated γh2ax
MGMT fusions protect from TMZ induced damage. a Clonogenic survival assay of U251 clones expressing MGMT fusions exposed to O 6 -BG (100μM) or/and TMZ (100μM) for 12 days. U251sgMSH6 cells are shown as control for TMZ resistance independently from MGMT. b Cell cycle distribution of U251 MGMT fusion expressing cells in presence of O 6 -BG (100μM) or/and TMZ (100μM) for 72h, measured by propidium iodide (PI) staining and FACS. U251 sgCtrl and sgMSH6 are shown as control. c High-throughput microscopy mediated quantification of cell cycle distribution at 48h after treatment. See methods for details. d Quantification of the percentage of cells in (c). Data are from a representative experiment repeated in triplicate and presented as mean (technical replicate n=3) and standard deviation. e - f High-throughput microscopy mediated quantification of <t>γH2AX</t> intensity levels and 53BP1 foci in U251 cells expressing the MGMT fusions after 48h of treatment with 100μM of the indicated drugs. U251sgCtrl and sgMSH6 were included as controls. Data are representative of three independent experiments. Student’s t test: *** P < 0.001, ** P < 0.01, * P < 0.05, ns = not significant; A.U., arbitrary unit.
Phosphorylated γh2ax, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated γh2ax/product/Enzo Biochem
Average 90 stars, based on 1 article reviews
phosphorylated γh2ax - by Bioz Stars, 2026-02
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anti-γ-h2ax antibody  (Cell Biolabs Inc)
90
Cell Biolabs Inc anti-γ-h2ax antibody
MGMT fusions protect from TMZ induced damage. a Clonogenic survival assay of U251 clones expressing MGMT fusions exposed to O 6 -BG (100μM) or/and TMZ (100μM) for 12 days. U251sgMSH6 cells are shown as control for TMZ resistance independently from MGMT. b Cell cycle distribution of U251 MGMT fusion expressing cells in presence of O 6 -BG (100μM) or/and TMZ (100μM) for 72h, measured by propidium iodide (PI) staining and FACS. U251 sgCtrl and sgMSH6 are shown as control. c High-throughput microscopy mediated quantification of cell cycle distribution at 48h after treatment. See methods for details. d Quantification of the percentage of cells in (c). Data are from a representative experiment repeated in triplicate and presented as mean (technical replicate n=3) and standard deviation. e - f High-throughput microscopy mediated quantification of <t>γH2AX</t> intensity levels and 53BP1 foci in U251 cells expressing the MGMT fusions after 48h of treatment with 100μM of the indicated drugs. U251sgCtrl and sgMSH6 were included as controls. Data are representative of three independent experiments. Student’s t test: *** P < 0.001, ** P < 0.01, * P < 0.05, ns = not significant; A.U., arbitrary unit.
Anti γ H2ax Antibody, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-γ-h2ax antibody/product/Cell Biolabs Inc
Average 90 stars, based on 1 article reviews
anti-γ-h2ax antibody - by Bioz Stars, 2026-02
90/100 stars
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anti-p-histone h2ax (γh2ax; phospho-ser139) antibody  (Beijing Solarbio Science)
90
Beijing Solarbio Science anti-p-histone h2ax (γh2ax; phospho-ser139) antibody
MGMT fusions protect from TMZ induced damage. a Clonogenic survival assay of U251 clones expressing MGMT fusions exposed to O 6 -BG (100μM) or/and TMZ (100μM) for 12 days. U251sgMSH6 cells are shown as control for TMZ resistance independently from MGMT. b Cell cycle distribution of U251 MGMT fusion expressing cells in presence of O 6 -BG (100μM) or/and TMZ (100μM) for 72h, measured by propidium iodide (PI) staining and FACS. U251 sgCtrl and sgMSH6 are shown as control. c High-throughput microscopy mediated quantification of cell cycle distribution at 48h after treatment. See methods for details. d Quantification of the percentage of cells in (c). Data are from a representative experiment repeated in triplicate and presented as mean (technical replicate n=3) and standard deviation. e - f High-throughput microscopy mediated quantification of <t>γH2AX</t> intensity levels and 53BP1 foci in U251 cells expressing the MGMT fusions after 48h of treatment with 100μM of the indicated drugs. U251sgCtrl and sgMSH6 were included as controls. Data are representative of three independent experiments. Student’s t test: *** P < 0.001, ** P < 0.01, * P < 0.05, ns = not significant; A.U., arbitrary unit.
Anti P Histone H2ax (γh2ax; Phospho Ser139) Antibody, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-p-histone h2ax (γh2ax; phospho-ser139) antibody/product/Beijing Solarbio Science
Average 90 stars, based on 1 article reviews
anti-p-histone h2ax (γh2ax; phospho-ser139) antibody - by Bioz Stars, 2026-02
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anti akt  (Boster Bio)
90
Boster Bio anti akt
MGMT fusions protect from TMZ induced damage. a Clonogenic survival assay of U251 clones expressing MGMT fusions exposed to O 6 -BG (100μM) or/and TMZ (100μM) for 12 days. U251sgMSH6 cells are shown as control for TMZ resistance independently from MGMT. b Cell cycle distribution of U251 MGMT fusion expressing cells in presence of O 6 -BG (100μM) or/and TMZ (100μM) for 72h, measured by propidium iodide (PI) staining and FACS. U251 sgCtrl and sgMSH6 are shown as control. c High-throughput microscopy mediated quantification of cell cycle distribution at 48h after treatment. See methods for details. d Quantification of the percentage of cells in (c). Data are from a representative experiment repeated in triplicate and presented as mean (technical replicate n=3) and standard deviation. e - f High-throughput microscopy mediated quantification of <t>γH2AX</t> intensity levels and 53BP1 foci in U251 cells expressing the MGMT fusions after 48h of treatment with 100μM of the indicated drugs. U251sgCtrl and sgMSH6 were included as controls. Data are representative of three independent experiments. Student’s t test: *** P < 0.001, ** P < 0.01, * P < 0.05, ns = not significant; A.U., arbitrary unit.
Anti Akt, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti akt/product/Boster Bio
Average 90 stars, based on 1 article reviews
anti akt - by Bioz Stars, 2026-02
90/100 stars
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histone h2ax [p ser139] antibody (9f3)  (Bio-Techne corporation)
90
Bio-Techne corporation histone h2ax [p ser139] antibody (9f3)
MGMT fusions protect from TMZ induced damage. a Clonogenic survival assay of U251 clones expressing MGMT fusions exposed to O 6 -BG (100μM) or/and TMZ (100μM) for 12 days. U251sgMSH6 cells are shown as control for TMZ resistance independently from MGMT. b Cell cycle distribution of U251 MGMT fusion expressing cells in presence of O 6 -BG (100μM) or/and TMZ (100μM) for 72h, measured by propidium iodide (PI) staining and FACS. U251 sgCtrl and sgMSH6 are shown as control. c High-throughput microscopy mediated quantification of cell cycle distribution at 48h after treatment. See methods for details. d Quantification of the percentage of cells in (c). Data are from a representative experiment repeated in triplicate and presented as mean (technical replicate n=3) and standard deviation. e - f High-throughput microscopy mediated quantification of <t>γH2AX</t> intensity levels and 53BP1 foci in U251 cells expressing the MGMT fusions after 48h of treatment with 100μM of the indicated drugs. U251sgCtrl and sgMSH6 were included as controls. Data are representative of three independent experiments. Student’s t test: *** P < 0.001, ** P < 0.01, * P < 0.05, ns = not significant; A.U., arbitrary unit.
Histone H2ax [P Ser139] Antibody (9f3), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/histone h2ax [p ser139] antibody (9f3)/product/Bio-Techne corporation
Average 90 stars, based on 1 article reviews
histone h2ax [p ser139] antibody (9f3) - by Bioz Stars, 2026-02
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Image Search Results


MGMT fusions protect from TMZ induced damage. a Clonogenic survival assay of U251 clones expressing MGMT fusions exposed to O 6 -BG (100μM) or/and TMZ (100μM) for 12 days. U251sgMSH6 cells are shown as control for TMZ resistance independently from MGMT. b Cell cycle distribution of U251 MGMT fusion expressing cells in presence of O 6 -BG (100μM) or/and TMZ (100μM) for 72h, measured by propidium iodide (PI) staining and FACS. U251 sgCtrl and sgMSH6 are shown as control. c High-throughput microscopy mediated quantification of cell cycle distribution at 48h after treatment. See methods for details. d Quantification of the percentage of cells in (c). Data are from a representative experiment repeated in triplicate and presented as mean (technical replicate n=3) and standard deviation. e - f High-throughput microscopy mediated quantification of γH2AX intensity levels and 53BP1 foci in U251 cells expressing the MGMT fusions after 48h of treatment with 100μM of the indicated drugs. U251sgCtrl and sgMSH6 were included as controls. Data are representative of three independent experiments. Student’s t test: *** P < 0.001, ** P < 0.01, * P < 0.05, ns = not significant; A.U., arbitrary unit.

Journal: bioRxiv

Article Title: MGMT genomic rearrangements contribute to chemotherapy resistance in gliomas

doi: 10.1101/2020.03.10.985226

Figure Lengend Snippet: MGMT fusions protect from TMZ induced damage. a Clonogenic survival assay of U251 clones expressing MGMT fusions exposed to O 6 -BG (100μM) or/and TMZ (100μM) for 12 days. U251sgMSH6 cells are shown as control for TMZ resistance independently from MGMT. b Cell cycle distribution of U251 MGMT fusion expressing cells in presence of O 6 -BG (100μM) or/and TMZ (100μM) for 72h, measured by propidium iodide (PI) staining and FACS. U251 sgCtrl and sgMSH6 are shown as control. c High-throughput microscopy mediated quantification of cell cycle distribution at 48h after treatment. See methods for details. d Quantification of the percentage of cells in (c). Data are from a representative experiment repeated in triplicate and presented as mean (technical replicate n=3) and standard deviation. e - f High-throughput microscopy mediated quantification of γH2AX intensity levels and 53BP1 foci in U251 cells expressing the MGMT fusions after 48h of treatment with 100μM of the indicated drugs. U251sgCtrl and sgMSH6 were included as controls. Data are representative of three independent experiments. Student’s t test: *** P < 0.001, ** P < 0.01, * P < 0.05, ns = not significant; A.U., arbitrary unit.

Article Snippet: Phospho-histone H2AX (Ser139) (γH2AX, Merck, Cat. 05-363, 1:1000) and 53BP1 (Novus Biologicals, Cat. NB100-304, 1:3000) immunofluorescence was performed using standard procedures.

Techniques: Clonogenic Cell Survival Assay, Clone Assay, Expressing, Staining, High Throughput Screening Assay, Microscopy, Standard Deviation

a Top panel : scheme of the in vivo experimental design. Bottom panel : Kaplan-Meier survival curve of animals intracranially injected with U251 sgCtrl and U251 BTRC-MGMT clone 2 cells transduced with a luciferase construct, treated or not with TMZ (50mg/Kg) for 5 days. sgCtrl Log-rank P value = 0.0049, BTRC-MGMT Log-rank P value = 0.9273. b Representative luminescent images of the tumor bearing at the indicated time points. c Immunohistochemistry analysis against BrdU and γH2AX of tumors from mice injected with U251 sgCtrl and BTRC-MGMT clone 2 cells, treated or not with TMZ (50mg/kg) for 3 days. Mice were sacrificed 2h after BrdU injection. Scale bars: 100μm. d Western blot analysis of the EXO markers Alix and Tgs101 and of MGMT levels in samples pair of cells and cell-derived EXOs expressing sgCtrl and SAR1A-MGMT. e SAR1A-MGMT and MGMT mRNA expression by RT–PCR in RNA pair samples from cells and cell derived EXOs expressing sgCtrl and SAR1A-MGMT. f Transcript levels of BTRC-MGMT by RT-PCR analysis in EXOs isolated from serum of BTRC-MGMT clone 2 tumor bearing mice compared to sgCtrl mice. U251sgCtrl and BTRC-MGMT clone 2 cells were included as controls.

Journal: bioRxiv

Article Title: MGMT genomic rearrangements contribute to chemotherapy resistance in gliomas

doi: 10.1101/2020.03.10.985226

Figure Lengend Snippet: a Top panel : scheme of the in vivo experimental design. Bottom panel : Kaplan-Meier survival curve of animals intracranially injected with U251 sgCtrl and U251 BTRC-MGMT clone 2 cells transduced with a luciferase construct, treated or not with TMZ (50mg/Kg) for 5 days. sgCtrl Log-rank P value = 0.0049, BTRC-MGMT Log-rank P value = 0.9273. b Representative luminescent images of the tumor bearing at the indicated time points. c Immunohistochemistry analysis against BrdU and γH2AX of tumors from mice injected with U251 sgCtrl and BTRC-MGMT clone 2 cells, treated or not with TMZ (50mg/kg) for 3 days. Mice were sacrificed 2h after BrdU injection. Scale bars: 100μm. d Western blot analysis of the EXO markers Alix and Tgs101 and of MGMT levels in samples pair of cells and cell-derived EXOs expressing sgCtrl and SAR1A-MGMT. e SAR1A-MGMT and MGMT mRNA expression by RT–PCR in RNA pair samples from cells and cell derived EXOs expressing sgCtrl and SAR1A-MGMT. f Transcript levels of BTRC-MGMT by RT-PCR analysis in EXOs isolated from serum of BTRC-MGMT clone 2 tumor bearing mice compared to sgCtrl mice. U251sgCtrl and BTRC-MGMT clone 2 cells were included as controls.

Article Snippet: Phospho-histone H2AX (Ser139) (γH2AX, Merck, Cat. 05-363, 1:1000) and 53BP1 (Novus Biologicals, Cat. NB100-304, 1:3000) immunofluorescence was performed using standard procedures.

Techniques: In Vivo, Injection, Transduction, Luciferase, Construct, Immunohistochemistry, Western Blot, Derivative Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation